Obokata calls the procedure "stimulus-triggered acquisition of pluripotency", and the resulting cells Stap cells. Worried about what regulators would think of mouse feeders, her lab switched to human feeders (Int J Mol Sci, 14:4684-704, 2013). Here, The Scientist profiles several approaches for avoiding MEFs, or ditching feeders altogether.
Scientists agree that for the clinic, products must be “xeno-free,” lacking in any components from nonhuman animals. Lucie Germain cultures skin cells to make grafts for burn patients. Any tissues grown from Stap cells would need to be proved safe in the body. The cells are divided into small colonies or single cells and transferred into new culture dish when the large colonies of embryonic stem cells are grown. Human bone marrow stem cells are isolated from the bone marrow and grown in culture media supplemented with serum from the blood. One of the many remaining questions is why cells in the body are not constantly turning into stem cells when they come into contact with acid, with heart burn, or when people drink fruit juice. It is necessary to grow stem cells in an undifferentiated state, and to make the cells differentiate into specialized cells, when required. Pijuan-Galitó has succeeded in culturing 16 different mouse and human stem cell lines with IαI. "So far they appear to be healthy, fertile, and normal," she said. Different culture medium is used to grow and maintain different types of stem cells. The feat has been hailed as … i need a stem cell transplant for c.i.d.p. Mouse feeders, in contrast, lift off the flask floor after a week or so. Hovatta’s collaborators founded a company, Biolamina of Stockholm (in which Hovatta also holds a stake), that sells diverse forms of laminin for €45–69 (US$51–79) per 100 micrograms. Obokata said the new procedure might have uses beyond regenerating damaged body parts, and shed light on the way cells gather wear and tear through our lifetimes.
However, feeders also create complications, forcing scientists to culture not one but two finicky cell types, then separate them later when the time comes to harvest the stem cells for analysis.... One popular option is to switch to Matrigel, a protein goo derived from cancerous mouse cells. The feat has been hailed as a major discovery by researchers familiar with the work, and if it can be repeated in human tissue, could lead to cheap and simple procedures to make patient-matched stem cells that could repair damaged or diseased organs.
Fasting.
they have done such cases sussessfully. Dr. Adria Schmedthorst.
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