These results highlight the difficulty associated with interpreting experimental results based on CFU, especially if treatments differ in DOM concentrations. For example, the human pathogen Vibrio cholerae is able to respond to host environmental signals by activating transcriptional regulatory cascades. Proc Natl Acad Sci U S A. Relationship between growth rate and CFU count for V. cholerae in carbon-amended treatments (▿) and other treatments (•) in microcosm C. Growth rate values were determined for the initial 24 h of each cycle from direct counts (as in Materials and Methods), and the CFU values are for the 24-h time point in each cycle.
Halophilic. Appl Environ Microbiol. Recolonization and ROS resistance of….
Vibrio cholerae interactions with the gastrointestinal tract: lessons from animal studies. Laboratory Methods for the Diagnosis of Vibrio cholerae Centers for Disease Control and Prevention Mix the suspension and antiserum well and then tilt slide back and forth to observe for agglutination. One factor that may provide insight into predicting infectivity routes is understanding whether infectivity is growth rate dependent. 7), which was itself experiencing positive growth. Davies BW, Bogard RW, Dupes NM, Gerstenfeld TA, Simmons LA, Mekalanos JJ. Much of the research emphasis has been on the importance of attachment and growth of V. cholerae to pelagic plankton, especially copepod zooplankters. Significance was determined as highly significant (P < 0.001) and significant (P < 0.05); P values of >0.05 were considered not significant. doi: 10.1371/journal.ppat.1001295. performed research; Z.Z. Rahul's Noteblog Notes on Bacteriology Notes on Growth Medias and Oxygen Requirements. Circles: dark green, treatment B; brown, treatment F; yellow, treatment TM; light green, treatment V. Values were determined by direct counts (see Table 1). If V. cholerae is capable of phenotypic plasticity similar to that of other marine and estuarine bacteria with respect to DOM-stimulated growth, then DOM availability may play an important role in regulating V. cholerae's population dynamics in the natural environment. To dissect the molecular mechanisms utilized by V. cholerae to overcome ROS in vivo, we performed a whole-genome transposon sequencing analysis (Tn-seq) by comparing gene requirements for colonization … Duplicate microcosms were inoculated with V. cholerae N16961 (VCRT). Strain and inoculum preparation. Colonies were counted after incubation for 12 h at 37°C in the dark.
In the nonamended treatment, V. cholerae showed low or negative growth rates. Epub 2013/01/24. We recognize the potential of colloidal organic matter to support bacterial growth, and inclusion of this material would probably have supported faster growth. Indole-positive . Bacterial abundance.Samples were fixed with formalin (2% final).
Microcosm experiment B. In addition, it has been found to attach not only to copepods (20, 25) but also to a variety of algal cells (21, 26). Vibrio oxygen use. 2002 Sep;3(2):29-38.
The growth rate was calculated as that occurring in the first 24 h of each cycle based on changes in cell abundance between endpoint samples (i.e., T0 and T24), based on the assumption of exponential growth, using the equation: μ (day−1) = ln(T24/T0).
3). We found that mutants of the methyl-directed mismatch repair (MMR) system, such as MutS, displayed significant colonization advantages in untreated, ROS-rich mice, but not in NAC-treated mice. Microcosm B was designed to provide a nonbloom water contrast for the growth of V. cholerae during low-enrichment periods. The “biological meaning” of the VBNC state has been debated for some time (see, for example, references 8, 31, and 44). All images were exposed for 100 ms and are shown at 1,000× magnification. Error bars represent the standard deviations of counts from duplicate treatments. NOTE: We only request your email address so that the person you are recommending the page to knows that you wanted them to see it, and that it is not junk mail. wrote the paper.
We tested the proliferation of V. cholerae N16961 during a Lingulodinium polyedrum bloom, as well as other seawater conditions.
(43) found that V. cholerae O1 had a significantly higher degree of binding to whole phytoplankton cells and detritus than to whole copepods in Bangladesh fresh waters. Vibrio cholerae serotype O1 is autochthonous to estuarine and coastal waters. This article contains supporting information online at www.pnas.org/lookup/suppl/doi:10.1073/pnas.1014640108/-/DCSupplemental. If applicable to the complex conditions in marine ecosystems, our results suggest an important role of the growth of free-living V. cholerae in disease propagation and prevention during phytoplankton blooms.
19 The toxR gene encodes a transcriptional activator controlling CT gene expression (ctxA), TCP biogenesis (tcpA), outer membrane protein expression (ompU), and at least 17 distinct genes in …
10.1038/nrmicro2204 . 2 and 4), provide another key insight into the ecology of this organism.
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