Please see the Microscope Dovetails tab or here for further details. Light from the sample plane can be collected via cameras, photomultiplier tubes (PMTs), or custom setups using breadboard tops. II. On the Cerna microscope, the objective is threaded onto a nosepiece, which holds the objective at the throat depth, or the distance from the optical path to the support rail of the microscope body. The epi-illumination module included with this microscope contains a turret that can hold up to six filter sets. Figure 1-5 Nomarski Differential Interference Contrast microscopy. Modules are attached to the microscope body via a circular dovetail (see the Microscope Dovetails tab or here for details). Manual translating platforms are also available via the full web presentation. The size of the throat depth, along with the working height, determine the working space available for microscopy. A D3T female dovetail on the rear of the microscope accepts a wide range of white-light lamps. Please see the full web presentation for the entire line of Thorlabs' filter sets. Nosepiece: A type of arm used to hold the microscope objective in the optical path of the microscope.
Unlike competing microscopes with similar capabilities, the Cerna platform's modularity lets the user quickly install and remove the microscope modules as needed for each experiment, providing a high degree of access and control. Trans-illumination modules, which condition light (on certain models) and direct it along the optical path, are attached to the support rail of the microscope body via a linear dovetail (see Microscope Dovetails tab or here).
Details can be found on the Microscope Add-Ons tab. Differential Interference Contrast Microscopy. Differential interference contrast (DIC) converts gradients in specimen optical path length into amplitude differences that can be visualized as improved contrast in the resulting image. Items may be removed from the default item list by changing the value in the "Qty" box to 0 before clicking the "Add Kit" button. Several filter sets aimed at common fluorophores are available below. Also described are the quantitative aspects of DIC imaging. Also included in the article is a drawing of the DIC microscope optical train and a section highlighting some of the most recent reviews on the topic. The rigid stands can translate by way of motorized stages (sold separately), while the mounting platforms contain built-in mechanics for motorized or manual translation. The Solis LED light sources have multiple wavelength emitting options, including broad sprectrum emission throughout the visible range.
Brightfield, differential interference contrast (DIC), Dodt gradient contrast, and darkfield microscopy are some examples of imaging modalities that utilize trans-illumination. Zeitschrift für Wissenschaftliche Mikroskopie und Mikroskopische Technik 69: 193-221 (1969). The specimen presented in Figure 2(a) is a doughnut-shaped human erythrocyte imaged at high magnification in differential interference contrast with the shear axis indicated by a double-headed arrow (northwest to southeast). For convenience, we stock several widefield Nikon objectives that are commonly used with Cerna microscopes, shown in the table below on the left. ¦ The 95 mm vertical support rail is the backbone of the Cerna microscope; modeled from Thorlabs' Precision 95 mm Optical Rails, it provides stable long-term support and excellent vibrational damping. Thorlabs offers various light sources for epi- and trans-illumination. A discussion of the microscope configuration for phase contrast and DIC with emphasis on similarities and differences between the complementary techniques. If you would like to order a different objective, please contact us. Differential Interference Contrast How DIC works, Advantages and Disadvantages. For more traditional sample mounting, slides can also be mounted directly onto the microscope body via a manual XY stage. See the comprehensive list below for each included item. Please see the, Visualize the Field of View on a Computer, Any C-Mount Camera is Compatible with a Cerna Microscope, White Light Sources Illuminate the Field of View Through the Objective, Light is Tuned by Filter Sets for Specific Fluorophores (See Below), Broadband LEDs Generate Intense Illumination Across the Visible Spectrum, Maximum LED Output Powers from 3.1 to 7.1 W, Combine 6 User-Changeable, High-Power LEDs into One Liquid Light Guide, Eleven Wavelength Options Cover All Known Fluorophores Between 365 nm and 780 nm (, Control LEDs Using Included Software with GUI, Typical Lifetime of LED Modules is ≥10,000 Hours. This brief review focuses on the history of DIC microscopy and provides an excellent set of early references that founded the technique. The arm attaches to a focusing module, used for aligning the condenser with respect to the sample and trans-illumination module. Please see the full web presentation of each to determine its functionality within the Cerna microscopy platform. Example imaging modalities include fluorescence, confocal, and reflected light microscopy. Click here for additional information about the Cerna microscope body. ZEISS Microscopy A discount is offered when a sufficient number of components are purchased.
1). Bellows: A tube with accordion-shaped rubber sides for a flexible, light-tight extension between the microscope body and the objective. Refer to Figure 1-5, B for the following discussion of differential interference contrast microscopy. Our in-stock selection is not exhaustive. Please note that our translating platform comes in both imperial and metric versions. ¦ Cerna® Microscope for Epi-Fluorescence and DIC with XY Platform, Motorized Translating XY Platform, 1/4"-20 Taps, LWD Condenser, 0.78 NA, Male D3N Dovetail, 400 - 850 nm, One C32 Tray Included, Epi-Illuminator Module with Turret for Six Filter Sets, Conditioning Optics, Additional Optical Port, WD = 50 mm, Motorized Module with 1" Travel for Edge-Mounted Arms, Motorized Module with 1" Travel for Middle-Mounted Arms, Cerna Microscope Body with Epi-Illumination Arm, 400 mm Rail, Cerna Trinoculars with 10X Eyepieces, Upright Image, IR Filter, 1X Camera Tube for LAURE1 & LAURE2 Trinoculars, C-Mount, Male D2N Dovetail, Variable Magnification Double Camera Port, Three-Channel Controller and Knob Box for 2" Cerna Travel Stages, Nosepiece for 2 Objectives, M25 x 0.75 Threads, Mounting Arm for CSN1201 and CSN1202 Nosepieces, Condenser Arm, ±2.5 mm Travel per Adjuster, Female D3N Dovetail, Three-Channel Controller and Knob Box for 1" Cerna Travel Stages, Transmitted Light Illumination / DIC Imaging Module, 30 mm Cage Compatible, 95 mm Dovetail Clamp for WFA1000 and WFA1100 Modules, Tray for Use with WFA3130 N1 DIC Prism or C4X Lens, T-Cube LED Driver, 1200 mA Max Drive Current (Power Supply Not Included), 15 V, 2.4 A Power Supply Unit with 3.5 mm Jack Connector for One K- or T-Cube, Nikon FN-C LWD Condenser, 0.78 NA, Male D3N Dovetail, Motorized Translating XY Platform, M6 x 1.0 Taps, Microscopes & DIY Components: Cerna Series, Illumination Sources for Epi-Illumination, Kit 4: Six-Position Epi Turret & Brightfield, Kit 6: Six-Position Epi Turret, Dodt, & Translator, Cerna Microscope Body with Epi-Illumination Arm, 400 mm Tall, Epi-Illuminator Module for Six Filter Sets (Filter Sets Not Included), Translating Platform with 2" Travel in Both X and Y, 3-Axis Controller for Translation, 2" Travel, Compact Condenser Mounting Arm with ±2.5 mm Travel per Adjuster, Motorized Focusing Module with 1" Travel for Edge-Mounted Arms, Motorized Focusing Module with 1" Travel for Middle-Mounted Arms, Brightfield Illumination / DIC Imaging Module, 15 V Power Supply Unit for a Single K-Cube™ or T-Cube™, On Semi / Truesense KAI-0340 Monochrome CCD, XY Platform Translates Large Apparatuses Over 2" Range in Both X and Y, Six-Position Epi-Illuminator and DIC Modules Support Visible and NIR Imaging, Large and Open Working Space Underneath the Objective, Ideal for Sample Apparatuses, Recording Chambers, and Micromanipulators, Compatible with Thorlabs' and Major Manufacturers' Objectives, Scientific Cameras, Fluorescence Filters, and Illumination Sources, Modular Design Allows User to Modify the Microscope's Optical Path, Supports DIC Imaging and Brightfield Illumination in the Visible and NIR, Motorized Condenser Focusing Module with 1" Travel, Move Rigid Stands, Micromanipulators, and Other Experimental Equipment Together Simultaneously, Imperial Version: 1/4"-20 Tapped Holes on 1" Centers, Metric Version: M6 x 1.0 Tapped Holes on 25 mm Centers, Large Working Volume: Optical Path is 7.74" (196.5 mm) Away from Edge of Rail, Linear Dovetail Surface Allows Modules to be Added and Removed, 400 mm Body Height Provides Space for DIC Imaging Module, Motorized Objective Focusing Module with 1" Travel, Variable-Magnification Double Camera Port for Independent Visible and NIR Cameras, Trinoculars with 10X Magnification and Adjustable Interpupil Distance, Use Low Magnification to Find the Region of Interest, then High Magnification to Image, Compatible with M25 x 0.75-Threaded Objectives (Nikon), Compatible with RMS-Threaded Objectives (Olympus) by using an, This microscope kit does not include a DIC objective prism, because it is objective-specific. Images produced in DIC microscopy have a distinctive shadow-cast appearance, as if they were illuminated from a highly oblique light source originating from a single azimuth. Broadband emission makes it possible for the same microscope to stimulate fluorophores that have absorption wavelengths that are spectrally separated.
For alternate rail heights, see the full web presentation. This nosepiece is secured to a motorized focusing module, used for focusing the objective as well as for moving it out of the way for sample handling. Tune Epi-Illumination Source for the Excitation and Detection of Specific Fluorophores, Up to Six Filter Sets can be Installed Simultaneously, Utilize Fluorescence Filters from Other Major Manufacturers, Microscope Accepts M25 x 0.75-Threaded Objectives (Nikon), Matching DIC Objective Prisms (Sold Separately) are Required for DIC, DIC Polarizers, Condenser Prisms, and Analyzer are Included with Microscope Kit, Rigid Stands Hold Samples Underneath and Around the Objective, Designed for Slides, Petri Dishes, Well Plates, Recording Chambers, Micromanipulators, and Custom Inserts, Translation Stages with 1" of X and Y Travel Available, Fixed Arms Incorporate Fast XY Stage, Lens Tubes, and/or Cage Systems Directly into Optical Path, CSA1000: For Our MLS203-1 Fast XY Scanning Stage, CSA1001: For Ø1" Lens Tubes and 30 mm Cage Systems, CSA1002: For Ø2" Lens Tubes and 60 mm Cage Systems, Circular Hole Designed for Recording Chambers.
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