UC-MSC-CM was collected, and UC-MSC-Exo was isolated and identified (Additional Fig.
PubMed As shown in Fig. The care and use of animals were approved by the Committee of Animal Care and Use of Hubei Provincial Center for Food and Drug Safety Evaluation and Animal Experiment (Permit No: 20200101). The experimental data were normalized to endogenous housekeeping gene GAPDH with 2−ΔΔCt method. The authors declare that they have no competing interests. J Surg Res. To further evaluate the anti-inflammatory and anti-fibrotic effects of UC-MSCs, in vitro experiments were performed in HK2 cells (human proximal tubular epithelial cell line), NRK-52E cells (rat renal tubular epithelial cell line), and hrGECs (human renal glomerular endothelial cell line).
Induced differentiation ability and characteristic surface markers of UC-MSCs. Proc Natl Acad Sci U S A. Li JH, et al. However, scientists have discovered that the umbilical cord is in fact very rich in stem cells. *P < 0.05, **P < 0.01 vs control +UC-MSC group. The immunofluorescence staining revealed that Collagen IV was majorly expressed in glomerular and tubulointerstitium in the DN group, while the expression of Collagen IV was greatly reduced in DN + UC-MSC group (Fig. 2019;18(4):2574–82. Only our licensed partners are able to provide parents who wish to store their baby’s cord lining tissue with the assurance that their tissue can be used for therapies when needed. J Am Soc Nephrol. Specific pathogen-free male Sprague-Dawley rats (180–200 g; 6 weeks old) from Hubei provincial center for disease control and prevention (Wuhan, China) were used in this research.
Cord Lining Epithelial Cells come from the upper layers of the cord lining and have been differentiated into: 1) Skin Cells, from which 3-dimensional skin (we call this an organotypic skin equivalent) has been made Professor Andrew Burd and his team in Hong Kong have comprehensively characterised Cord Lining Epithelial Cells, clearly demonstrated their multipotent nature, and successfully constructed a three-dimensional organotypic skin equivalent. The UC-MSCs associated with markers were positive for CD105, CD90, CD44, and CD73 and were negative for CD19, CD34, CD45, and HLA-DR. To explore the therapeutic effect of UC-MSCs on DN, we established a rat model of DN induced by STZ injection. g, h Concentration of TGF-β in plasma (g) and kidney (h) tissues. If material is not included in the article's Creative Commons licence and your intended use is not permitted by statutory regulation or exceeds the permitted use, you will need to obtain permission directly from the copyright holder. Correspondence to Many researchers believe that the homing ability of MSCs may be the key to their therapeutic effect. We found that 2 weeks after UC-MSC transplantation, compared with the DN group, the 24-h urinary protein concentration, Scr, urea nitrogen, and renal hypertrophy index of the DN + UC-MSC group were significantly reduced, while Ccr was significantly increased, suggesting that UC-MSCs had a protective effect on kidney function. Human umbilical cord-derived mesenchymal stem cells prevent the progression of early diabetic nephropathy through inhibiting inflammation and fibrosis. 2016;25(2):313–26. a Blood glucose concentration curve.
PubMed Please check you selected the correct society from the list and entered the user name and password you use to log in to your society website. Related cell surface markers, such as CD19, CD34, CD45, HLA-DR, CD105, CD90, CD44, and CD73 (Biolegend, USA), were detected by flow cytometry. The inflammatory cell infiltration and interstitial fibrosis in DN are associated with multiple inflammatory cytokines, such as IL-1β, IL-6, TNF-α, and TGF-β [21, 34, 35]. Members of _ can log in with their society credentials below. Part of Cord lining progenitor cells: potential in vitro adipogenesis model.
The IP you are accessing the site with (192.169.201.024) has been blocked because it has triggered one of our security measures. View or download all the content the society has access to. Clin J Am Soc Nephrol. Rats were maintained under standard feeding conditions (temperature 20 ± 2 °C; humidity 45–55%; 12 h light & dark cycle) and free access to food and water. Epithelial stem cells can be differentiated into any type of epithelial cell such as skin cells, corneal conjunctival cells, liver cells, oesophageal cells, intestinal lining cells, as well as cells of the lung, airways, and bladder. 2018;215:113–8. Fiori A, et al. Diabetologia. 3f). These results indicated that UC-MSCs can home to kidney tissues and improve the symptoms and pathological changes of DN to a certain extent. Performed data analysis: E Xiang, Changyong Li, and Dongcheng Wu. Epub 2012 Nov 7. Compared with DN group, UC-MSC treatment obviously suppressed the increase in 24-h urinary protein (Fig. 2020;29:963689720904628.
Google Scholar. Particle size and concentrations were measured by nanoparticle tracking analysis (NTA) (Particle Metrix, Germany).
7a). Besides, the blood glucose levels of DN group were greater than 16.7 mmol/L and were significantly higher than that of the control rats (Fig. Wrote or contribution to the writing of the manuscript: E Xiang, Changyong Li, and Dongcheng Wu. 2c). Access to society journal content varies across our titles. Biol Blood Marrow Transplant. Thery C, et al. This result is consistent with the previous report [30], which may be due to the late treatment of MSCs and the missed opportunity for repairing the acute injury of the pancreas. Contact us if you experience any difficulty logging in. 2019;104(1–2):71–80. 4c). Patent Applications have been successful in numerous territories for processes of stem cell derivation as well as therapeutic uses. ( A ) VK2 cells were co-cultured with hucMSC, human fibroblasts (HFF-1) or KSFM medium in …
2b).
Death, end-stage renal disease and renal function decline in patients with diabetic nephropathy in French cohorts of type 1 and type 2 diabetes. For immunofluorescence staining, kidney paraffin slices were dewaxed into water. 2019;13(5):411–22. 3g). PubMed Quantifying levels of transplanted murine and human mesenchymal stem cells in vivo by real-time PCR. Montanari S, et al. 3d, e).
The incidence of DN can reach 30 to 40% after 20 years of diabetes, of which 5~10% of patients will progress to end-stage renal disease, and epidemiological surveys predict that by 2030, DN will become the seventh leading cause of death in the world [2,3,4,5]. Privacy d, e Immunofluorescence staining of F4/80 (d) and CD3 (e) in kidney tissues. The addition of Epithelial and Mesenchymal stem cells from its amniotic membrane strengthens its versatility. e-h Concentration of TGF-β (e), IL-6 (f), IL-1β (g), and TNF-α (h) in the supernatant of HK2 cells. Our patents cover “mammalian umbilical cord lining”, and we work on both human and animal derived stem cells. The 24-h urinary protein ≥ 30 mg/24 h was verified as DN. UC-MSCs are becoming another promising MSCs for treating a variety of human refractory diseases. 1). Human umbilical cord MSCs (Huc-MSCs) are active throughout pregnancy. 4). The concept and the epidemiology of diabetic nephropathy have changed in recent years. About 100 mg of the kidney tissue was lysed in 1 mL ice-cold RIPA lysis buffer (Beyotime, China) with 1 mM PMSF. https://doi.org/10.1186/s13287-020-01852-y, DOI: https://doi.org/10.1186/s13287-020-01852-y. Characterization of human umbilical cord- lining derived epithelial cells and transplantation potential. Stem Cells Int. Paracrine effect of mesenchymal stem cell as a novel therapeutic strategy for diabetic nephropathy. All cord lining tissue banked with our partners will be included in the Global Cord Registry, and only tissue registered therein will be legally available for therapies.
8i, UC-MSCs secreted large amounts of EGF, FGF, HGF, and VEGF in both UC-MSC-CM and UC-MSC-Exo. These results indicated that UC-MSCs effectively improved renal function in DN rats. b The number of UC-MSCs detected in the kidneys of DN rats. G: glomerulus. To further confirm that TGF-β was involved in renal fibrosis protection, RT-qPCR, ELISA, and Western blot assays were performed. dopamine in patients with Parkinson’s disease). To detect the degree of pathological damage and fibrosis of kidney tissues, hematoxylin and eosin (H&E), periodic acid-Schiff (PAS), and Masson’s trichrome (Masson) staining were used to observe the morphological changes of the kidneys under a light microscope. The control group was assayed as a negative control. 2012;380(9854):1662–73. The Masson’s trichrome staining of renal tissues showed that renal glomerular and interstitial fibrosis occurred in the DN group, while the renal fibrosis was improved by UC-MSC treatment in DN + UC-MSC group (Fig. Mitochondria transfer from mesenchymal stem cells structurally and functionally repairs renal proximal tubular epithelial cells in diabetic nephropathy in vivo. Moreover, UC-MSCs secreted large amounts of growth factors including epidermal growth factor, fibroblast growth factor, hepatocyte growth factor, and vascular endothelial growth factor. Open Access This article is licensed under a Creative Commons Attribution 4.0 International License, which permits use, sharing, adaptation, distribution and reproduction in any medium or format, as long as you give appropriate credit to the original author(s) and the source, provide a link to the Creative Commons licence, and indicate if changes were made.
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