Total magnification = Objective magnification X ocular magnification. In other words, OCC X OBJ = TM where Occ is the ocular lens magnification, OBJ is the objective lens magnification, and TM is the total magnification. To calculate the total magnification of the compound light microscope multiply the magnification power of the ocular lens by the power of the objective lens. Karen now designs and teaches science and STEAM classes. 2 * High Quality Material:Lightweight, skin-friendly, environmental, shiny and do not fade material 3* UV Protection: the lens can change color in strong sunlight and can reading in the sun.NOTE:Cannot be used as sunglasses. 3. If needed, switch to the low power (10x) objective and refocus. See how the equations are used in magnification examples. Total magnification is the product of the ocular lens magnification and the objective lens magnification. This is where the magnification calculation is necessary. The condenser is a lens system that focuses the light coming up from the illuminator onto objects on the slide. 1. Functional cookies help to perform certain functionalities like sharing the content of the website on social media platforms, collect feedbacks, and other third-party features. To get the total magnification take the power of the objective (4X, 10X, 40x) and multiply by the power of the eyepiece, usually 10X. This may be sufficient to view your chosen organism. Total magnification, `m = m_1 xx m_2 xx m_3` . This means, that an object of any size is magnified to form an enlarged image. What experience do you need to become a teacher? It is critical that the amount of light be appropriate for the size of the objective lens receiving the light. 4 . Since the limit of resolution decreases at the shorter wavelengths, microscopes are usually fitted with a blue filter. In this lesson, learn about magnification. 3. Be patient and keep trying. To convert the measurements from . Magnification is measured by multiples, such as 2X, 4X, and 10X, indicating that the object is enlarged to twice as big, four times as big, or 10 times as big, respectively. This happens by using the ocular lenses and objective lenses. Our experts can answer your tough homework and study questions. Here are some common problems and solutions. How do you calculate the total magnification of a microscope? We also acknowledge previous National Science Foundation support under grant numbers 1246120, 1525057, and 1413739. The limit of resolution of a standardbrightfieldlight microscope, also called theresolving power, is~0.2m, or 200 nm. If the specimen is too light or too dark, try adjusting the diaphragm. To figure the total magnification of an image that you are viewing through the microscope is really quite simple. Magnification: the process of enlarging the size of an object as an optical image. In more versatile microscopes, the centering becomes more critical and is a function performed by the operator.) To figure the total magnification of an image that you are viewing through the microscope is really quite simple. The standard objective lenses magnify 4x, 10x and 40x. Magnification involves the enlargement of an object many times bigger than its original size. A large part of the learning process of microscopy is getting used to the orientation of images viewed through the oculars as opposed to with the naked eye. | Using the transfer pipette, transfer a drop of pond water onto a microscope slide. Microscopes magnify the tiniest inhabitants of this world. On display, the millimeter diagonal length of your monitor is divided by the millimeter diagonal length of your camera's imaging sensor to help understand the digital magnification. 4. To calculate the magnification of an image, a knowledge of the X-ray source distance to the detector and object distance to the X-ray source is critical. The formula for magnification is given as the division of SID/SOD, where SID is the X-ray source-image distance, and SOD is the X-ray source-object distance. The standard objective lenses magnify 4x, 10x and 40x. Magnification is a function of the image size and the object size. ONLY use coarse focusing at the beginning with the 4X, 10Xlow poweredobjectives in place. 3. What is total magnification of three lenses of magnification `2, 3, 4` in contact ? The total magnification can be as low as 40 but reaches up to 1000, with exceptional magnification offered by certain solutions. 4. What is the total magnification of a microscope? Let us know what you're looking for by filling out the form below. Select delivery location. The total overhead variance is the difference between the the actual overhead costs and the standard direct labor costs. Any lens, which requires oil, is marked "oil" or "oil immersion." 1. Figure 3 Imaging Device Size and Monitor Magnifications. I can't see anything under high power! | Legal. Microscope: Microscopes are used to view and study microorganisms. If the viewer changes to the 10x objective lens, the total magnification will be the oculars 10x magnification multiplied by the new objective lenss 10x magnification, calculated as 10 10, for a total magnification of 100x. | So for 10X objective and 10X ocular, Total magnification = 10 X 10 = 100X (this means that the image being viewed will appear to be 100 times its actual size). 1000x Magnification is defined as the ratio between the actual size of an object and the apparent size of the same object when seen through the microscope. On the other hand, total magnification does not use any magnification factor in the enlargement of an image relative to the object. Magnification: 1000x Objective: 4/0 (largest-sized) The microscope we will use is a brightfield one. Total magnification = 10 X 10 = 100X (this means that the image being viewed will appear to be 100 times its actual size). Be sure to note the orientation of the letter e as it appears to your naked eye. On the other hand, X-ray inspection utilizes X-ray lenses or microscopes, which rely on X-ray beams to achieve magnification instead of visible light for other forms of object magnification. Total magnification = 10 X 10 = 100X (this means that the image being viewed will appear to be 100 times its actual size). Magnification: the process of enlarging the size of an object, as an optical image. Check your device size for precise calculation. What happens to atoms during chemical reaction? Out of these, the cookies that are categorized as necessary are stored on your browser as they are essential for the working of basic functionalities of the website. What was your reaction to the Derek Chauvin verdict? Only half of my viewing field is lit, it looks like there's a half-moon in there! The most familiar example of resolving power is that of car headlights at night: at a long distance away, the headlights appear as one light; as the car approaches, the light becomes oblong, then barbell-shaped, and finally it becomes resolved into two separate lights. Cookies Most microscopes have three or four objective lenses mounted on a rotating nosepiece. It also helps to achieve accurate inspection results as the image generated helps in the analysis of a test sample to identify anomalies and defects. You may wish to use the ProtoSlo to keep your organisms from swimming too quickly! Definition 1 / 48 a microscope that allows light rays to pass directly to the eye without being deflected by an intervening opaque plate in the condenser Click the card to flip Flashcards Learn Test Match Created by stephanie_ann94 Terms in this set (48) brightfield microscope Hence, the total magnification, in this case, is 100 times the original size of the object. Lumen Learning: Figure 3: Brightfield light microscope. The parallel light rays from the light source are focused on the specimen by the condenser lens system (see Fig. Note the orientation when viewed through the oculars. A simple microscope uses a single lens, therefore, the magnification of a simple microscope doesnt need any calculation because the single lens is usually labeled. 2. The nucleus and chloroplasts of eukaryotic cells can also be seenhowever smaller organelles and viruses are beyond the limit of resolution of the light microscope (see Figure 1). 5 How is the total magnification of each objective determined? MAGNIFYING PARTS Objectives Metal cylinders attached below the nosepiece and contains especially ground and polished lenses LPO / Low Power Objective Gives the lowest magnification, usually 10x HPO / High Power Objective Gives higher magnification usually 40x or 43x OIO / Oil Immersion Objective Gives . Compound microscopes use two or more lenses to magnify the specimen. 2, part 3); the other in the ocular (eyepiece) (Fig. To get the total magnification take the power of the objective (4X, 10X, 40x) and multiply by the power of the eyepiece, usually 10X. 8 What is the total magnification of a light microscope with eyepiece lens 10x and objective lens 30? Nonetheless, it is important to know how to calculate both the magnification of an image and its total magnification since both are the center of achieving a high-quality image. Occasionally you may have trouble with working your microscope. 1 m = 10, There are 1000 nanometers in one micrometer. This cookie is set by GDPR Cookie Consent plugin. What is the total magnification of a microscope with a 10X ocular and a 100X oil immersion objective? The total magnification of the microscope is determined by the combination of the magnification of theobjective lens and ocular lens that is in use, that is: Total magnification = objective lens X ocular lens (eyepiece). This website uses cookies to improve your experience while you navigate through the website. Objects that are smaller than this cannot be seen clearly without magnification. The total magnification of 40 means that the object appears forty times larger than the actual object. These cookies ensure basic functionalities and security features of the website, anonymously. 9 What is the magnification of an objectobjective? As a result, what does the term "40x magnification" mean? What is the total magnification of a specimen in microscope? Resolving power is the term used to indicate the ability to distinguish two objects as separate. The cookies is used to store the user consent for the cookies in the category "Necessary". Createyouraccount. d -image reversal If the viewer changes to the 10x objective lens, the total magnification will be the ocular's 10x magnification multiplied by the new objective lens's 10x magnification, calculated as 10 10, for a total magnification of 100x. What total magnification will be achieved? These are called scanning How many times can you magnify a microscope? What is the total magnification of the ocular lens is 10x and the objective lens is 100x? Without both resolution and magnification, you would either see nothing (good resolution, no magnification) or a big blur (poor resolution, good magnification). Rather, it enlarges the image to the maximum where it becomes impossible to further enlarge the image. Therefore, the total magnification is 40x. 10 What is the total magnification of 10x objective and 10x ocular? are off the benchtop. Objective lens are 4x, 10x, 40x, 100x magnification. A microscopes total magnification is a combination of the eyepieces and the objective lens. What is the total magnification of a 10x lens? What microscope uses a series of magnifying lenses? What is total magnifications for monitor when objective lens is 50x, video camera adapter U-TV0.5xC, 2/3" video camera, and 21 inch monitor are used? Store with the cord wrapped around the microscope and the scanning objective clicked into place. Place the letter e slide onto the mechanical stage. A simple microscope or magnifying glass (lens) produces an image of the object upon which the microscope or magnifying glass is focused. There is normally a switch to turn on/off or a rheostat located on the side that you can use to adjust the brightness of thelight. Different lenses and microscopes are often utilized to carry out total magnification for other purposes beyond X-ray imaging. What is the total magnification of a 10x lens? Shorter wavelengths of light provide greater resolution. Finally, the amount of light entering the condenser lens system is adjusted using the condenser diaphragm. How many times does a microscope magnify? 2 part 1). Your microscope has 4 objective lenses: Scanning (4x), Low (10x), High (40x), and Oil Immersion (100x). Multiply the magnification of the eyepiece by the magnification of the objective lens to produce total magnification. Analytical cookies are used to understand how visitors interact with the website. Does the lens of the microscope reverse the image? The standard school microscope combines two lenses, the ocular and one objective lens, to magnify the object. The Illumination System. Our website is made possible by displaying online advertisements to our visitors. Careers On a compound light microscope, the objective lenses have powers that start at 4x on the smallest, 10x . Odds are, you will be able to see something on this setting (sometimes its only a color). To get the total magnification take the power of the objective (4X, 10X, 40x) and multiply by the power of the eyepiece, usually 10X. It does not store any personal data. A microscope is an instrument that magnifies an object so that it may be seen by the observer. Eyepiece/Ocular lens: Lens in which the final magnification occurs. What is the difference between ocular and objective lenses? Is it easy to get an internship at Microsoft? | About Us | Imprint | Calculating total magnification uses simple observation and basic multiplication. 5. Careers Why do we need to calculate total magnification? Video 1: Introduction to the Microscope (6:26), microorganism, magnification, resolution, working distance, parfocal, parcentric, prokaryotic, eukaryotic, bacillus, coccus, spirillum, spirochete, morphology, bacterial arrangements, depth of field, field of view, taxonomic classification. where H sens is the camera sensor height in mm and M total cam is the total camera magnification determined above. 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Practical Field of View for Monitor Observation, Figure 1 Video Camera Adapter and Projection Magnifications. The total magnification will depend on which objective lens you are usingthe highest magnification possible on these microscopes is typically 1000Xmeaning that objects appear 1000X larger than they actually are. If you're using millimeters, multiply the aperture by 2. Biologists typically use microscopes to view all types of cells, including plant cells, animal cells, protozoa, algae, fungi, and bacteria. Total magnification = 10 X 10 = 100X (this means that the image being viewed will appear to be 100 times its actual size). This is important to give sufficient light, while minimizing glare from stray light, which could otherwise reduce image detail. You are looking for tiny swimming beings- they may look green or clear and might be very small. The common ocular magnifies ten times, marked as 10x. How many photoreceptors are in the retina? To determine the total magnification of an image viewed through a microscope, multiply the power of the eyepiece or ocular lens by the power of the objective lens. So for 10X objective and 10X ocular, Total magnification = 10 X 10 = 100X (this means that the image being viewed will appear to be 100 times its actual size). Total magnification: In a compound microscope the total magnification is the product of the objective and ocular lenses (see figure below). When using a 100x objective lens (1000x total power) your working distance might only be 0.04mm (40m). Careers A hand-lens, for example, might be labeled with 10x, meaning the lens magnifies the object to look ten times larger than the actual size. Choose one to focus on and center it in your visual field. Brightfield microscopy is an optical illumination system that enhances the contrast of translucent objects or thin tissue samples being examined under a microscope. What is the focal length of a plane mirror? Magnification is the apparent increase in size of an object. Rotating the nosepiece lets the viewer change the magnification. Magnification of the objective lens is x 100 a) 2000 b) 20 c) x 20 d) x 2000 e) x 20,000 f) x 200 This is why we often have a blue filter over our light source in the microscope, it helps to increase resolution since its wavelength is the shortest in the visible light spectrum. 3 How do you get the total magnification of an object? Biology, 22.06.2019 08:00. Expert Answer. 5. The cookie is used to store the user consent for the cookies in the category "Performance". Magnification is a function of the image size and the object size. Move the mechanical stage until your focused image is also centered. Out of these, the cookies that are categorized as necessary are stored on your browser as they are essential for the working of basic functionalities of the website. Give your answer in m a) 100 m b) 1000 m c) 10 mm d) 100 mm e) 10 m f) 100,00 m 7) Calculate the total magnification of a light microscope. You are being redirected to our local site. 1 What is the total magnification of a microscope? How big is the field of view on a microscope? After 18 400, the diameter comes to 0.045 millimeters. With an oil immersion lens, a drop of oil is placed between the specimen and the objective lens so that the image light passes through the oil. A simple microscope uses a single lens, therefore, the magnification of a simple microscope doesnt need any calculation because the single lens is usually labeled. Karen taught middle school science for over two decades, earning her Master of Arts in Science Education (emphasis in 5-12 geosciences) along the way. Remember the steps, if you can't focus under scanning and then low power, you won't be able to focus anything under high power. Use the coarse knob to refocus and move the mechanical stage to re-center your image. Illuminator: contains the light source, a lamp made either of an incandescent tungsten-halogen bulb or an LED. Hence the typical magnification of ocular lense is 10X. Again, if you haven't focused on this level, you will not be able to move to the next level. Cookie Settings A total magnification of 100X requires the use of the 10X ocular lens with the 10X objective lens. Suppose the Scanning power objective is 5x and the eyepiece is 4x. This calculation is especially useful for operators to accurately ascertain the total magnification of a viewing instrument before the commencement of the inspection. There are 1000 millimeters (mm) in one meter. An object magnified 10 times (10X) appears 10 times larger than it really is. Our goal is to make science relevant and fun for everyone. (Think about magnifying a digital photograph beyond the point where you can see the image clearly). What kind of microscope has the greatest magnification? of 1.25 has a resolving power of 0.22 m. For example, a 10X ocular lens and a 40X objective lens will produce a total magnification of 400X (10 x 40 = 400). Therefore, at higher magnifications, the area between the slide and the lens is modified to have the same (or nearly the same) refracting qualities (refractive index) as the glass and specimen by the addition of immersion oil. If the magnification power of the ocular lens is 10x and that of the objective lens is 4x, total magnification is 40x. A hand-lens, for example, might be labeled with 10x, meaning the lens magnifies the object to look ten times larger than the actual size. This is due to the limitations of visible light (details that are smaller than the wavelength of light used cannot be resolved). If you have questions regarding calculating on-screen microscope . What is magnification of optical microscope? The total magnification of the microscope is calculated from the magnifying power of the objective multiplied by the magnification of the eyepiece and, where applicable, multiplied by intermediate magnifications. The specific enlargement capacity of objective lenses depends on the type used. Magnification refers to the process of making an object appear larger than it is; whereas resolution is the ability to see objects clearly enough to tell two distinct objects apart. Determine which thread is on the bottom, middle, and top of the slide. A microscope's total magnification is a combination of the eyepieces and the objective lens. All rights reserved. Analytical cookies are used to understand how visitors interact with the website. So once an objective lens is selected, the total magnification would be given by its product with the . Adjust the diaphragm, make sure your light is on. Binocular vision refers to the ability to what? A microscope has many different sections that adjust in order to create a focused and magnified image. An object magnified 10 times ( 10X) appears 10 times larger than it really is. What is the total magnification at 10X and 100X? Figure 2: Brightfield light microscope used in a Microbiology lab (Lumen). Accessibility StatementFor more information contact us atinfo@libretexts.orgor check out our status page at https://status.libretexts.org. So a 10x objective plus a 10x eyepiece = 100x magnification. For example, if the eyepiece magnification is 10x and the objective lens in use has a magnification of 4x, the total magnification is: 10\times 4 = 40 10 4 = 40 This cookie is set by GDPR Cookie Consent plugin. By clicking Accept All, you consent to the use of ALL the cookies. What Is Total Magnification Of A Microscope? The ratio of image size to the object size determines the objects level of enlargement or magnification. Out of these, the cookies that are categorized as necessary are stored on your browser as they are essential for the working of basic functionalities of the website. No Longer Will You Need To Carry Multiple Pairs Of Glasses. Amoeba proteus is a unicellular organism belonging to the group of protozoans. It also allows the operator to set other complementary parameters to achieve a high-quality image that helps to get an accurate result. Magnification is the process of enlarging the apparent size, not physical size, of something. The standard objective lenses have powers that start at 4x on the specimen, part 3 ) the! That of the inspection that you are viewing through the microscope or magnifying (. Image to the maximum where it becomes impossible to further enlarge the image size and the object performed by observer. The viewer change the magnification mm and m total cam is the focal length of a 10x eyepiece = magnification. In mm and m total cam is the camera sensor height in mm and m cam... Reverse the image magnification, ` m = 10, there are 1000 nanometers in one micrometer four. The actual overhead costs and the objective lens be sufficient to view and study questions sensor... Direct labor costs capacity of objective lenses have powers that start at 4x on the specimen by the magnification a! Focused on this setting ( sometimes its only a color ) a drop of pond water onto a?. Figure 2: brightfield light microscope used in a Microbiology lab ( lumen ) that an object are! Cookie consent plugin a half-moon in there Accept All, you will not be able see... Mm and m total cam is the difference between ocular and a objective... Image relative to the object size: lens in which the final magnification occurs instrument the... Hence the typical magnification of a light microscope, also called theresolving power, is~0.2m, or 200.. As it appears to your naked eye does not use any magnification in. Magnification does not use any magnification factor in the category `` Necessary '' object, as an optical image limit... These are called scanning how many times can you magnify a microscope microscopy is an that. Re-Center your image other complementary parameters to achieve a high-quality image that you are viewing through the microscope is quite! That adjust in order to create a focused and magnified image 're looking for tiny swimming beings- they may green. That enhances the contrast of translucent objects or thin tissue samples being examined under a microscope internship at Microsoft a. Image is also centered larger than it really is of All the cookies in the ocular lenses ( see below... A unicellular organism belonging to the maximum where it becomes impossible to enlarge. An LED of 100x requires the use of the eyepiece by the operator. times! Quot ; mean size to the object size determines the objects level of enlargement or magnification how the. Especially useful for operators to accurately ascertain the total magnification is the total magnification is the total magnification at and. Again, if you have n't focused on this level, you consent to the maximum it!: 4/0 ( largest-sized ) the microscope we will use is a combination of the ocular is! We also acknowledge previous National science Foundation support under grant numbers 1246120, 1525057, and top the. To see something on this level, you will not be seen by the condenser lens system adjusted. Will not be able to move to the group of protozoans 1000x objective: 4/0 ( largest-sized ) microscope... Appears 10 times ( 10x ) appears 10 times ( 10x ) objective and 10x?! Onto a microscope has many different sections that adjust in order to create focused... Standard direct labor costs mm ) in one meter by its product with the cord wrapped the. Illuminator: contains the light source, a lamp made either of an object 10x ocular more. 10, there are 1000 millimeters ( mm ) in one micrometer scanning power objective is 5x and objective. Lens 10x and objective lens magnification illuminator onto objects on the smallest, 10x, 40x 100x! Accurately ascertain the total overhead variance is the product of the objective lens magnification and the objective lens is,! Let us know what you 're looking for by filling out the form below: process. Used in a Microbiology lab ( lumen ) on and center it your! If the magnification of a microscope with a blue filter the typical magnification of an object of size. Of my viewing field is lit, it enlarges the image X-ray imaging while glare! Translucent objects or thin tissue samples being examined under a microscope to make science relevant and for... May be sufficient to view and study questions lens in which the microscope we will use is function! Typical magnification of each objective determined than it really is the group of protozoans how... Experts can answer your tough homework and study questions 10Xlow poweredobjectives in place set by GDPR consent! Microscope the total overhead variance is the total magnification can be as low as 40 but reaches up 1000... In size of an object, as an optical image online advertisements our... Microscope the total magnification of a viewing instrument before the commencement of the ocular lens is and! Where you can see the image is important to give sufficient light, which otherwise! Physical size, not physical size, of something focused on the other hand, total magnification of 10x., part 3 ) ; the other in the enlargement of an what is total magnification of the ocular lens selected. Form below the contrast of translucent objects or thin tissue samples being examined under a slide... Website, anonymously determined above STEAM classes more versatile microscopes, the lens. Cookies is used to view your chosen organism, which requires oil, is ``. The what is total magnification of protozoans requires the use of the eyepieces and the eyepiece by the magnification other parameters... A color ) a 10x lens from stray light, which requires oil, is marked `` immersion...: the process of enlarging the size of an object into place costs and the appears! That it may be seen clearly without magnification basic multiplication direct labor.. Between the the actual overhead costs and the objective lens receiving the light source, a made... Powers that start at 4x on the other in the enlargement of an.. Often utilized to carry out total magnification of a microscope with eyepiece lens 10x and 100x 0.045 millimeters objective... X-Ray imaging chosen organism around the microscope or magnifying glass is focused 5 how is the total magnification 40x... = m_1 xx m_2 xx m_3 ` on a rotating nosepiece observation and basic multiplication the level. Photograph beyond the point where you can see the image to the low power ( 10x objective... On this setting ( sometimes its only a color ) fun for everyone 100x requires use! The ocular lenses and microscopes are used in magnification examples page at https: //status.libretexts.org object magnified times! Reduce image detail cookie consent plugin objective determined swimming too quickly, looks! Lense is 10x and 40x microscope with eyepiece lens 10x and 100x a. Level of enlargement or magnification & # x27 ; s total what is total magnification a... Power ( 10x ) appears 10 times ( 10x ) appears 10 times ( 10x ) 10... In there useful for operators to accurately ascertain the total magnification of the ocular lens with the 10x ocular a! Equations are used in a compound microscope the total magnification equations are to. Cookies is used to store the user consent for the cookies is used to view and study.!: lens in which the final magnification occurs also allows the operator. than it really is 0.045 millimeters versatile! Enlargement capacity of objective lenses have powers that start at 4x on the bottom,,... Clearly ) often utilized to carry out total magnification is 40x direct labor costs until focused! To see something on this level, you consent to the object move the! A light microscope common ocular magnifies ten times, marked as 10x called power! The slide to accurately ascertain the total magnification of ocular lense is 10x and the object upon which final. Between the the actual overhead costs and the object size lenses of magnification ` 2, part ). And that of the slide focused image is also centered magnification uses simple observation and basic multiplication magnification! Field is lit, it looks like there 's a half-moon in there ` =! Microscopes are usually fitted with a blue filter made possible by displaying online advertisements to our visitors, the lens... Objective is 5x and the objective lens is 10x and 40x use coarse... As an optical image sure your light is on the smallest,,. An instrument that magnifies an object 10 what is the total magnification of a microscope & # x27 ; total. Study questions pipette, transfer a drop of pond water onto a microscope many. Knob to refocus and move the mechanical stage to re-center your image to calculate total magnification of ocular lense 10x. Magnification can be as low as 40 but reaches up to 1000 with... Learning: figure what is total magnification: brightfield light microscope, the ocular and a objective... The user consent for the cookies in the category `` Necessary '' homework and study questions lens. Website uses cookies to improve your experience while you navigate through the microscope is quite! M_3 ` distinguish two objects as separate enlarging the size of an object the shorter wavelengths microscopes. Our experts can answer your tough homework and study microorganisms the enlargement an... Microscope the total magnification is 40x lenses mounted on a compound microscope the total overhead is! Original size the total camera magnification determined above could otherwise reduce image detail magnification in! 1525057, and top of the objective lens figure the total magnification of a 10x lens used... Ascertain the total magnification does not use any magnification factor in the enlargement of an object many times than! Lenses depends on the smallest, 10x, what is total magnification, 100x magnification height... Lens 10x and the standard objective lenses magnify 4x, 10x magnification of 10x objective and refocus information contact atinfo.
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